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New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata

Received: 15 October 2021     Accepted: 4 November 2021     Published: 9 December 2021
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Abstract

Background: Caloncoba echinata is used in traditional medicine as vomiting, against lice and mange as well as in the treatment of dermal infection, leprosy, pustular eruption (small-pox) [1, 4]. It has been reported that the non-edible vegetable oil from Caloncoba echinata seeds possess potent antibacterial activity on Escherichia coli and Staphylococcus aureus [5]. Objective: This work addressed the phytochemical investigation of the methanolic extract of leaves of Caloncoba echinate. Both extracts and all the isolates were screened for the antibacterial activities. Method: All the compounds were characterized by spectroscopic and mass spectrometric methods, and by comparison with literature data. The antibacterial activity of both extract and some isolated compounds against bacteria was determined using broth microdilution method in 96-well microtitre sterile plates as previously described [11]. Results: From the methanolic crude extract of the leaves of Caloncoba echinate, a new derivative flavonol named Kaempferol-4',7-dimethoxy-3-O-(6"-O-acetyl)-β-NULL-glucopyranoside (1) together with nine known compounds namely ermanin-3-O-β-D glucopyranoside (2), Kaempferol-4′,7-dimethoxy-3-O-(3",4",6"-O-triacetyl)-β-NULL glucopyranoside (3), friedelan-3-one (4), 29-hydroxyfriedelan-3-one (5), mixture of β-sitosterol and stigmasterol (6-7), mixture of β-sitosterol and stigmasterol glucoside (8-9), Lupeol (10) were isolated. Furthermore, compounds (2) and (5) were reported here for the first time from the Caloncoba genus. Crude extract exhibited a significant activity against the five bacteria with the MIC = 62.5µg/mL for Salmonella typhi, Escherishia coli, Shigella flexineri and the MIC = 32.25µg/mL for Salmonella typhimurium, S. enteritidis. For the isolated compounds, the best activities were recorded by compound (1) showing a moderate activity against Salmonella typhi (MIC = 32.25µg/mL; MBC/MIC = 8), Salmonella typhimurium (62.5µg/mL; MBC/MIC = 4), Salmonella enteritidis (62.25 µg/mL; MBC/MIC = 2). Conclusion: These results showed that the antimicrobial activities could be mainly attributed to the constituents of flavonol glycoside (1). In addition, the antibacterial bioactivities and determined constituents support the use of this specie by traditional healers to treat a certain number of bacterial diseases.

Published in Science Journal of Chemistry (Volume 9, Issue 6)
DOI 10.11648/j.sjc.20210906.14
Page(s) 155-159
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Achariaceae, Caloncoba echinate, Flavonol, Antimicrobial Activities

References
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    Charlemagne Ndoumbe Tamba, Sergi Herve Akone, Caroline Ngo Nyobe, Claudia Stevine Popwo Tameye, Jean Pierre Longue Ekon, et al. (2021). New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata. Science Journal of Chemistry, 9(6), 155-159. https://doi.org/10.11648/j.sjc.20210906.14

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    Charlemagne Ndoumbe Tamba; Sergi Herve Akone; Caroline Ngo Nyobe; Claudia Stevine Popwo Tameye; Jean Pierre Longue Ekon, et al. New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata. Sci. J. Chem. 2021, 9(6), 155-159. doi: 10.11648/j.sjc.20210906.14

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    AMA Style

    Charlemagne Ndoumbe Tamba, Sergi Herve Akone, Caroline Ngo Nyobe, Claudia Stevine Popwo Tameye, Jean Pierre Longue Ekon, et al. New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata. Sci J Chem. 2021;9(6):155-159. doi: 10.11648/j.sjc.20210906.14

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  • @article{10.11648/j.sjc.20210906.14,
      author = {Charlemagne Ndoumbe Tamba and Sergi Herve Akone and Caroline Ngo Nyobe and Claudia Stevine Popwo Tameye and Jean Pierre Longue Ekon and Jules Lobe Songue and Jean Claude Ndom},
      title = {New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata},
      journal = {Science Journal of Chemistry},
      volume = {9},
      number = {6},
      pages = {155-159},
      doi = {10.11648/j.sjc.20210906.14},
      url = {https://doi.org/10.11648/j.sjc.20210906.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20210906.14},
      abstract = {Background: Caloncoba echinata is used in traditional medicine as vomiting, against lice and mange as well as in the treatment of dermal infection, leprosy, pustular eruption (small-pox) [1, 4]. It has been reported that the non-edible vegetable oil from Caloncoba echinata seeds possess potent antibacterial activity on Escherichia coli and Staphylococcus aureus [5]. Objective: This work addressed the phytochemical investigation of the methanolic extract of leaves of Caloncoba echinate. Both extracts and all the isolates were screened for the antibacterial activities. Method: All the compounds were characterized by spectroscopic and mass spectrometric methods, and by comparison with literature data. The antibacterial activity of both extract and some isolated compounds against bacteria was determined using broth microdilution method in 96-well microtitre sterile plates as previously described [11]. Results: From the methanolic crude extract of the leaves of Caloncoba echinate, a new derivative flavonol named Kaempferol-4',7-dimethoxy-3-O-(6"-O-acetyl)-β-NULL-glucopyranoside (1) together with nine known compounds namely ermanin-3-O-β-D glucopyranoside (2), Kaempferol-4′,7-dimethoxy-3-O-(3",4",6"-O-triacetyl)-β-NULL glucopyranoside (3), friedelan-3-one (4), 29-hydroxyfriedelan-3-one (5), mixture of β-sitosterol and stigmasterol (6-7), mixture of β-sitosterol and stigmasterol glucoside (8-9), Lupeol (10) were isolated. Furthermore, compounds (2) and (5) were reported here for the first time from the Caloncoba genus. Crude extract exhibited a significant activity against the five bacteria with the MIC = 62.5µg/mL for Salmonella typhi, Escherishia coli, Shigella flexineri and the MIC = 32.25µg/mL for Salmonella typhimurium, S. enteritidis. For the isolated compounds, the best activities were recorded by compound (1) showing a moderate activity against Salmonella typhi (MIC = 32.25µg/mL; MBC/MIC = 8), Salmonella typhimurium (62.5µg/mL; MBC/MIC = 4), Salmonella enteritidis (62.25 µg/mL; MBC/MIC = 2). Conclusion: These results showed that the antimicrobial activities could be mainly attributed to the constituents of flavonol glycoside (1). In addition, the antibacterial bioactivities and determined constituents support the use of this specie by traditional healers to treat a certain number of bacterial diseases.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - New Bioactive Flavonoid Derivative from the Leaves of Caloncoba echinata
    AU  - Charlemagne Ndoumbe Tamba
    AU  - Sergi Herve Akone
    AU  - Caroline Ngo Nyobe
    AU  - Claudia Stevine Popwo Tameye
    AU  - Jean Pierre Longue Ekon
    AU  - Jules Lobe Songue
    AU  - Jean Claude Ndom
    Y1  - 2021/12/09
    PY  - 2021
    N1  - https://doi.org/10.11648/j.sjc.20210906.14
    DO  - 10.11648/j.sjc.20210906.14
    T2  - Science Journal of Chemistry
    JF  - Science Journal of Chemistry
    JO  - Science Journal of Chemistry
    SP  - 155
    EP  - 159
    PB  - Science Publishing Group
    SN  - 2330-099X
    UR  - https://doi.org/10.11648/j.sjc.20210906.14
    AB  - Background: Caloncoba echinata is used in traditional medicine as vomiting, against lice and mange as well as in the treatment of dermal infection, leprosy, pustular eruption (small-pox) [1, 4]. It has been reported that the non-edible vegetable oil from Caloncoba echinata seeds possess potent antibacterial activity on Escherichia coli and Staphylococcus aureus [5]. Objective: This work addressed the phytochemical investigation of the methanolic extract of leaves of Caloncoba echinate. Both extracts and all the isolates were screened for the antibacterial activities. Method: All the compounds were characterized by spectroscopic and mass spectrometric methods, and by comparison with literature data. The antibacterial activity of both extract and some isolated compounds against bacteria was determined using broth microdilution method in 96-well microtitre sterile plates as previously described [11]. Results: From the methanolic crude extract of the leaves of Caloncoba echinate, a new derivative flavonol named Kaempferol-4',7-dimethoxy-3-O-(6"-O-acetyl)-β-NULL-glucopyranoside (1) together with nine known compounds namely ermanin-3-O-β-D glucopyranoside (2), Kaempferol-4′,7-dimethoxy-3-O-(3",4",6"-O-triacetyl)-β-NULL glucopyranoside (3), friedelan-3-one (4), 29-hydroxyfriedelan-3-one (5), mixture of β-sitosterol and stigmasterol (6-7), mixture of β-sitosterol and stigmasterol glucoside (8-9), Lupeol (10) were isolated. Furthermore, compounds (2) and (5) were reported here for the first time from the Caloncoba genus. Crude extract exhibited a significant activity against the five bacteria with the MIC = 62.5µg/mL for Salmonella typhi, Escherishia coli, Shigella flexineri and the MIC = 32.25µg/mL for Salmonella typhimurium, S. enteritidis. For the isolated compounds, the best activities were recorded by compound (1) showing a moderate activity against Salmonella typhi (MIC = 32.25µg/mL; MBC/MIC = 8), Salmonella typhimurium (62.5µg/mL; MBC/MIC = 4), Salmonella enteritidis (62.25 µg/mL; MBC/MIC = 2). Conclusion: These results showed that the antimicrobial activities could be mainly attributed to the constituents of flavonol glycoside (1). In addition, the antibacterial bioactivities and determined constituents support the use of this specie by traditional healers to treat a certain number of bacterial diseases.
    VL  - 9
    IS  - 6
    ER  - 

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Author Information
  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Thermal and Energetic Engineering, Douala Institute of Technology, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

  • Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon

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